Antibodies directed against guanylic acid and guanosine are nucleolus specific probes

Iswari, S and Krishnamurthy, DS and Jacob, TM (1985) Antibodies directed against guanylic acid and guanosine are nucleolus specific probes. In: FEBS letters, 189 (2). pp. 296-300.

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Abstract

Purified antibodies against guanylic acid and guanosine binding to RNA at guanosine residues were used to probe human lymphocyte preparations by indirect immunofluorescence. Neither antibody gave any banding pattern with metaphase chromosomes but both showed binding to specific sites in the interphase nuclei. Evidence presented indicates that these sites are guanosine residues on rDNA transcripts at the nucleolar organizer regions.

Item Type:	Journal Article
Publication:	FEBS letters
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier.
Keywords:	Indirect immunofluorescence;Guanylic acid antibody;Human lymphocyte;Guanosine binding.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	16 Jul 2008
Last Modified:	19 Sep 2010 04:47
URI:	http://eprints.iisc.ac.in/id/eprint/14933

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