A rapid method of epitope analysis using Superose 12 gel filtration a study with monoclonal antibodies to chicken riboflavin carrier protein

Karande, Anjali A and Visweswariah, Sandhya S and Adiga, Radhakantha P (1987) A rapid method of epitope analysis using Superose 12 gel filtration a study with monoclonal antibodies to chicken riboflavin carrier protein. In: Journal of Immunological Methods, 99 (2). pp. 173-177.

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Abstract

A novel and rapid method is described for determining the epitope specificites of monoclonal antibodies. The method utilises a highly reproducible, wide range gel filtration medium, Superose 12. nmol of monoclonal antibodies are incubated with pmol quantities of radiolabelled antigen, and the mixture filtered through Superose 12. Fractions are collected and radioactivity monitored. The size of the resultant immune complex indicates whether two monoclonal antibodies (mixed with the antigen) recognise the same or different epitope(s) of the antigen. The applicability of the above analytical method is illustrated with monoclonal antibodies raised against chicken egg riboflavin carrier protein.

Item Type:	Journal Article
Publication:	Journal of Immunological Methods
Publisher:	Elsevier Science
Additional Information:	Copyright for this article belongs to Elsevier Science.
Keywords:	Epitope analysis;Superose gel filtration;Monoclonal antibody;Riboflavin carrier protein;chicken
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	13 May 2008
Last Modified:	01 Mar 2012 07:33
URI:	http://eprints.iisc.ac.in/id/eprint/13954

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