Solubilization of bilirubin by cholate micelles. Spectroscopic and gel permeation studies

Puranam, Kasturi L and Balaram, P (1987) Solubilization of bilirubin by cholate micelles. Spectroscopic and gel permeation studies. In: Proceedings of the Indian Academy of Sciences - Chemical Sciences, 98 (5-6). pp. 453-468.

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Abstract

The interactions of bilirubin with bile salts were studied by fluorescence, CD, and 1H NMR methods. Enhancement of bilirubin fluorescence and induction of optical activity in bilirubin in the presence of cholate has been obsd. Fluorescence enhancement is pronounced above the crit. micelle concn., whereas induced CD bands are detectable even in the premicellar region. Dehydrocholate and deoxycholate did not increase fluorescence, but did induce CD bands for bilirubin. Gel-permeation chromatog. on Sephadex G-50 yielded a single bilirubin-cholate species at alk. pH, whereas 2 species were obtained at neutral pH. 1H NMR and CD spectral characterizations of these complexes are reported. A 4:1 cholate-bilirubin mixt. was analyzed by difference NOE spectroscopy. Observation of strong, neg. NOE, both intermol. and intramol., suggests that specific Me groups on bilirubin and cholate are proximal in the mixed micelle.

Item Type:	Journal Article
Publication:	Proceedings of the Indian Academy of Sciences - Chemical Sciences
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright belongs to Indian Academy of Sciences. The present publication is known as Journal of Chemical Sciences
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	29 Feb 2008
Last Modified:	19 Sep 2010 04:42
URI:	http://eprints.iisc.ac.in/id/eprint/13136

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