Glycosidic bond cleavage of 5-fluoro-2'-deoxyuridine and 5-fluorouridine by amino acyl-tRNA synthetases

Kumar, Madan A and Nayak, R (1990) Glycosidic bond cleavage of 5-fluoro-2'-deoxyuridine and 5-fluorouridine by amino acyl-tRNA synthetases. In: Biochemical and Biophysical Research Communications, 173 (2). pp. 731-735.

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Abstract

Lysyl-tRNA synthetase isolated from rat liver cleaves glycosidic bond of 5-Fluorouridine and 5-Fluoro-2'-deoxyuridine to generate 5-Fluorouracil. The generation of 5-Fluorouracil was monitored by cellulose thin layer chromatography and by spectrophotometry. The enzyme was found to be 1.4 fold more efficient in cleaving the glycosidic bond of 5-Fluoro-2'-deoxyuridine than 5-Fluorouridine.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Academic Press, Inc.
Additional Information:	Copyright of this article belongs to Academic Press, Inc.
Keywords:	Glycosidic bond cleavage;amino acyl-tRNA;chromatography;spectrophotometry
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	10 Dec 2007
Last Modified:	19 Sep 2010 04:41
URI:	http://eprints.iisc.ac.in/id/eprint/12477

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