Exploiting lectin affinity chromatography in clinical diagnosis

Satish, Posettihalli R and Surolia, Avadhesha (2001) Exploiting lectin affinity chromatography in clinical diagnosis. In: Journal of Biochemical and Biophysical Methods, 49 (1-3). pp. 625-640.

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Abstract

Lectin affinity chromatography (LAC) offers a tool that aids purification of cell surface glycoconjugates in sufficient quantities so that studies addressing their structural elucidation could be carried out. It has several advantages over the conventional biochemical methods, such as immunoprecipitation and/or immunoaffinity chromatography, used for the purification of various glycoconjugates. Serial LAC (SLAC) not only helps establish the identity of a glycoprotein or allows purification of a glycoprotein to homogeneity from among a mixture of glycoproteins, but it also successfully resolves the microheterogeneity in these glycoproteins, which is an otherwise impracticable problem to address. Specific cases of the altered expression and maintenance of microheterogeneity of some of the glycoproteins in pathological conditions vis a vis during normal biology are presented. The application of LAC in (i) itself, (ii) a serial fashion, and (iii) conjunction with other techniques such as two-dimensional electrophoresis, capillary electrophoresis, mass spectrometry, etc. in the diagnosis of certain pathological conditions, and the possibility of using this knowledge in designing treatments for various diseases, is discussed.

Item Type:	Journal Article
Publication:	Journal of Biochemical and Biophysical Methods
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier.
Keywords:	Lectin affinity chromatography;Serial lectin affinity chromatography;Glycoprotein microheterogeneity;Human chorionic gonadotropin;α-Foetoprotein;Mass spectrometry
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	16 Oct 2007
Last Modified:	19 Sep 2010 04:40
URI:	http://eprints.iisc.ac.in/id/eprint/12200

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