Structural Basis for Triclosan and NAD Binding to Enoyl-ACP Reductase of Plasmodium falciparum

Suguna, Kaza and Surolia, Avadhesha and Surolia, Namita (2001) Structural Basis for Triclosan and NAD Binding to Enoyl-ACP Reductase of Plasmodium falciparum. In: Biochemical and Biophysical Research Communications, 283 (1). pp. 224-228.

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Abstract

Recent discovery of type II fatty acid synthase in the malarial parasite Plasmodium falciparum responsible for the most debilitating form of the disease in humans makes it ideal as a target for the development of novel antimalarials. Also, the identification of the enoyl-acyl carrier protein reductase from P. falciparum and the demonstration of its inhibition by triclosan [5-chloro-2-(2,4-dichlorophenoxy)phenol], a potent antibacterial compound, provide strong support for the above. In the studies reported here, a model of the enzyme in complex with triclosan and the cofactor NAD has been built by homology modeling with a view to understand its binding properties and to explore the potential of triclosan as a lead compound in designing effective antimalarial drugs. The model indeed provided the structural rationale for its interaction with ligands and the cofactor and revealed unique characteristics of its binding site which could be exploited for improving the specificity of the inhibitors.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier.
Keywords:	Enoyl-ACP reductase;FabI;Plasmodium falciparum;Molecular model;Triclosan;NAD binding
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	05 Oct 2007
Last Modified:	19 Sep 2010 04:40
URI:	http://eprints.iisc.ac.in/id/eprint/12101

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