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Two internal ribosome entry sites mediate the translation of p53 isoforms

Ray, Partho Sarothi and Grover, Richa and Das, Saumitra (2006) Two internal ribosome entry sites mediate the translation of p53 isoforms. In: EMBO Reports, 7 (4). pp. 404-410.

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The p53 tumour suppressor protein has a crucial role in cell-cycle arrest and apoptosis. Previous reports show that the p53 messenger RNA is translated to produce an amino-terminal-deleted isoform $({\triangle}N-p53)$ from an internal initiation codon, which acts as a dominant-negative inhibitor of full-length p53. Here, we show that two internal ribosome entry sites (IRESs) mediate the translation of both full-length and ${\triangle}N-p53$ isoforms. The IRES directing the translation of full-length p53 is in the 5'-untranslated region of the mRNA, whereas the IRES mediating the translation of ${\triangle}N-p53$ extends into the protein-coding region. The two IRESs show distinct cell-cycle phase-dependent activity, with the IRES for full-length p53 being active at the G2–M transition and the IRES for ${\triangle}N-p53$ showing highest activity at the G1–S transition. These results indicate a novel translational control of p53 gene expression and activity.

Item Type: Journal Article
Additional Information: Copyright of this articles belongs to Nature Publishing Group.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Depositing User: Sahana R Sahini
Date Deposited: 31 May 2006
Last Modified: 27 Aug 2008 12:05
URI: http://eprints.iisc.ac.in/id/eprint/7171

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