Single-stranded DNA binding and methylation by EcoP1I DNA methyltransferase

Sistla, Srivani and Krishnamurthy, Vinita and Rao, Desirazu N (2004) Single-stranded DNA binding and methylation by EcoP1I DNA methyltransferase. In: Biochemical and Biophysical Research Communications, 314 (1). pp. 159-165.

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Abstract

EcoP1I methyltransferase (M.EcoP1I) belongs to the type III restriction–modification system encoded by prophage P1 that infects Escherichia coli. Binding of M.EcoP1I to double-stranded DNA and single-stranded DNA has been characterized. Binding to both single- and double-stranded DNA could be competed out by unlabeled single-stranded DNA. Metal ions did not influence DNA binding. Interestingly, M.EcoP1I was able to methylate single-stranded DNA. Kinetic parameters were determined for singleand double-stranded DNA methylation. This feature of the enzyme probably functions in protecting the phage genome from restriction by type III restriction enzymes and thus could be considered as an anti-restriction system. This study describing in vitro methylation of single-stranded DNA by the type III methyltransferase EcoP1I allows understanding of the mechanism of action of these enzymes and also their role in the biology of single-stranded phages.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
Additional Information:	The copyright belongs to Elsevier.
Keywords:	Anti-restriction;Binding;Methylation;Single-stranded DNA
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	31 May 2006
Last Modified:	19 Sep 2010 04:28
URI:	http://eprints.iisc.ac.in/id/eprint/7157

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