ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

One-pot bottom-up synthesis of a 2D graphene derivative: Application in biomolecular recognition and nanozyme activity

Pandit, S and De, M (2021) One-pot bottom-up synthesis of a 2D graphene derivative: Application in biomolecular recognition and nanozyme activity. In: Nanoscale Advances, 3 (17). pp. 5102-5110.

[img]
Preview
PDF
nan_adv_03-17_5102-5110_2021.pdf - Published Version

Download (816kB) | Preview
[img]
Preview
PDF
d1na00226k1.pdf - Published Supplemental Material

Download (668kB) | Preview
Official URL: https://doi.org/10.1039/d1na00226k

Abstract

The synthesis of two-dimensional (2D) nanosheets such as graphene and its derivatives through a bottom-up approach has many advantages such as growth control and functionalization, but it is always challenging to get the desired material. Herein, we have reported the synthesis of water soluble 2D-nanosheets through a bottom-up approach from 2,4,6-tribromo-3-hydroxybenzoic acid via a self-coupling pathway and characterized them using several techniques. AFM and TEM analyses reveal that the synthesized material has a layered structure with a thickness of �1.2 nm. Also, the prepared nanosheets are amorphous in nature with high negative charge (-38 ± 2.5 mV). The flexible nature of 2D-nanosheets and their functionality can be used in many related applications. Therefore, we have utilized the synthesized 2D-nanosheets in biomolecular recognition studies. It was found that the enzymatic activity of α-chymotrypsin can be controlled reversibly in the presence of the synthesized 2D-nanosheets. The kinetic study revealed that the nanosheet surface selectively binds to the active sites of the enzyme through a competitive pathway. Furthermore, we explored the nanozyme activity of the material in a peroxidase-like activity assay of two bio-active molecules: Nicotinamide Adenine Dinucleotide Phosphate (NADH) and dopamine. The results suggest that the prepared material efficiently catalyzed the oxidation of NADH to biological cofactor NAD+ and dopamine to aminochrome in the presence of H2O2. These synthesized graphene-like 2D-nanosheets with functional groups can be further tuned with other functionalities, which can open a new window for other related applications. © The Royal Society of Chemistry.

Item Type: Journal Article
Publication: Nanoscale Advances
Publisher: Royal Society of Chemistry
Additional Information: The copyright for this article belongs to Authors
Keywords: Amines; Graphene; Hydrogen peroxide; Molecular recognition; Neurophysiology, Alpha chymotrypsins; Bioactive molecules; Biomolecular recognition; Enzymatic activities; Nicotinamide adenine dinucleotide phosphates; Peroxidase-like activities; Synthesized materials; Two Dimensional (2 D), Nanosheets
Department/Centre: Division of Chemical Sciences > Organic Chemistry
Date Deposited: 02 Dec 2021 12:54
Last Modified: 02 Dec 2021 12:54
URI: http://eprints.iisc.ac.in/id/eprint/70070

Actions (login required)

View Item View Item