Mass Spectrometric Analysis of Cyclotides from Clitoria ternatea: Xxx-Pro Bond Fragmentation as Convenient Diagnostic of Pro Residue Positioning

Kalmankar, NV and Balaram, P and Venkatesan, R (2021) Mass Spectrometric Analysis of Cyclotides from Clitoria ternatea: Xxx-Pro Bond Fragmentation as Convenient Diagnostic of Pro Residue Positioning. In: Chemistry - An Asian Journal .

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Abstract

Cyclotides, a class of macrocyclic plant peptides, characterized by a cyclic backbone and three inter-locking disulfide bonds, may be divided into two major structural subfamilies, Möbius and Bracelet, based on the presence or absence of a specific proline residue. The present study describes the suite of cyclotides obtained from Clitoria ternatea, characterized by LC�MS and MS/MS techniques. Notable variations in product ion distributions were observed in cyclotides belonging to different structural subfamilies based on the number and positions of proline residues. For instance, Cter M which is an abundant Möbius cyclotide in this plant containing three proline residues, displayed distinct b- and y- ion characteristics in the MS/MS spectra compared to Cliotide T1, another commonly identified cyclotide but belonging to the Bracelet subfamily having two proline residues. The distinct fragmentation pattern of prototypical cyclotides of each structural subfamily, determined by Xxx-Pro bond fragmentation, was used to rapidly identify and sequence a novel cyclotide ctr pep 30 from this plant. © 2021 Wiley-VCH GmbH.

Item Type:	Journal Article
Publication:	Chemistry - An Asian Journal
Publisher:	John Wiley and Sons Ltd
Additional Information:	The copyright for this article belongs to John Wiley and Sons Ltd
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	28 Nov 2021 09:36
Last Modified:	28 Nov 2021 09:36
URI:	http://eprints.iisc.ac.in/id/eprint/69977

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