Genomic analysis of a multidrug-resistant Brucella anthropi strain isolated from a 4-day-old neonatal sepsis patient

Lama, M and Chanakya, PP and Khamari, B and Peketi, ASK and Kumar, P and Muddu, GK and Nagaraja, V and Bulagonda, EP (2021) Genomic analysis of a multidrug-resistant Brucella anthropi strain isolated from a 4-day-old neonatal sepsis patient. In: Journal of Global Antimicrobial Resistance, 26 . pp. 227-229.

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Abstract

Objectives: Brucella anthropi is a Gram-negative, aerobic, motile, oxidase-positive, non-fermentative Alphaproteobacteria belonging to the family Brucellaceae. It is most commonly found in soil but is an emerging, opportunistic, nosocomial human pathogen. The objective of this study was to understand the genome features of a drug-resistant B. anthropi (SOA01) isolated from a blood culture of a 4-day-old neonate and to determine its antimicrobial resistance and pathogenic potential. Methods: Hybrid genome assembly of B. anthropi strain SOA01 was generated using quality-trimmed short Illumina and long MinION reads. Identification and antimicrobial susceptibility profile were determined by MALDI-TOF, in silico ribosomal multilocus sequence typing (rMLST) and VITEK®2, respectively. PATRIC webserver and VFDB were used to identify antimicrobial resistance (AMR), virulence factor (VF) and transporter genes. Results: Multidrug-resistant B. anthropi strain SOA01 has a genome of 4 975 830 bp with a G+C content of 56.29. Several AMR, VF and transporter genes were identified in the genome. Antimicrobial susceptibility testing revealed resistance to different classes of antibiotics in strain SOA01. Conclusion: Brucella anthropi SOA01 is a multidrug-resistant strain. Several AMR and VF genes were identified in the genome, revealing the potential threat posed by this pathogen. The genome data generated in this study are likely to be useful in better understanding its AMR mechanisms, pathogenic potential and successful adaptation from its primary habitat of soil to the human system. Since it is often misidentified as Brucella melitensis or Brucella suis, genome characterisation and detailed understanding of its biology are crucial. © 2021 The Author(s)

Item Type:	Journal Article
Publication:	Journal of Global Antimicrobial Resistance
Publisher:	Elsevier Ltd
Additional Information:	The copyright for this article belongs to Authors
Keywords:	amikacin; amoxicillin plus clavulanic acid; ampicillin; cefepime; cefoperazone plus sulbactam; ceftriaxone; cefuroxime; cefuroxime axetil; ciprofloxacin; contig; cotrimoxazole; gentamicin; imipenem; meropenem; nalidixic acid; nitrofurantoin; piperacillin plus tazobactam; ribosome RNA; tigecycline; transfer RNA; untranslated RNA; virulence factor, ampicillin resistance; antibiotic resistance; antibiotic sensitivity; bacterial gene; bacterial strain; bacterial virulence; bacterium isolation; blood culture; Brucella; Brucella anthropi; clinical article; computer model; genome; genome analysis; genomic island; human; hybrid; matrix assisted laser desorption ionization time of flight mass spectrometry; mobile genetic element; multidrug resistant bacterium; multilocus sequence typing; newborn; newborn sepsis; nonhuman; Note; phylogenetic tree; prophage; pseudogene; reference database; transposon; whole genome sequencing
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	25 Sep 2021 12:38
Last Modified:	25 Sep 2021 12:38
URI:	http://eprints.iisc.ac.in/id/eprint/69789

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