Vinculin-mediated axon growth requires interaction with actin but not talin in mouse neocortical neurons

Mandal, P and Belapurkar, V and Nair, D and Ramanan, N (2021) Vinculin-mediated axon growth requires interaction with actin but not talin in mouse neocortical neurons. In: Cellular and Molecular Life Sciences, 78 . pp. 5807-5826.

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Abstract

The actin-binding protein vinculin is a major constituent of focal adhesion, but its role in neuronal development is poorly understood. We found that vinculin deletion in mouse neocortical neurons attenuated axon growth both in vitro and in vivo. Using functional mutants, we found that expression of a constitutively active vinculin significantly enhanced axon growth while the head-neck domain had an inhibitory effect. Interestingly, we found that vinculin-talin interaction was dispensable for axon growth and neuronal migration. Strikingly, expression of the tail domain delayed migration, increased branching, and stunted axon. Inhibition of the Arp2/3 complex or abolishing the tail domain interaction with actin completely reversed the branching phenotype caused by tail domain expression without affecting axon length. Super-resolution microscopy showed increased mobility of actin in tail domain expressing neurons. Our results provide novel insights into the role of vinculin and its functional domains in regulating neuronal migration and axon growth. © 2021, The Author(s), under exclusive licence to Springer Nature Switzerland AG.

Item Type:	Journal Article
Publication:	Cellular and Molecular Life Sciences
Publisher:	Springer Science and Business Media Deutschland GmbH
Additional Information:	The copyright for this article belongs to Springer Science and Business Media Deutschland GmbH
Department/Centre:	Division of Biological Sciences > Centre for Neuroscience
Date Deposited:	03 Aug 2021 11:22
Last Modified:	03 Aug 2021 11:22
URI:	http://eprints.iisc.ac.in/id/eprint/69033

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