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Biotin-Appended Iron(III) Complexes of Curcumin for Targeted Photo-Chemotherapy

Sahoo, S and Raghavan, A and Kumar, A and Nandi, D and Chakravarty, AR (2021) Biotin-Appended Iron(III) Complexes of Curcumin for Targeted Photo-Chemotherapy. In: European Journal of Inorganic Chemistry, 2021 (17). pp. 1640-1650.

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Official URL: https://doi.org/10.1002/ejic.202001174

Abstract

Iron(III) complexes of curcumin (Hcur) having a tridentate NNN-donor dipicolylamine-based ligand L1 and its biotinylated analogue L2, namely, Fe(L1)(cur)Cl (1) and Fe(L2)(cur)Cl (2), were prepared, characterized and their photo-induced cytotoxicity studied. The complexes exhibited curcumin-based absorption band near 430 nm and emission maxima at 520 nm. Complex 2 with a biotin moiety showed enhanced cellular uptake and higher cytotoxicity compared to its non-biotin analogue 1. The visible light induced cytotoxicity was studied in HeLa, MCF-7 and HepG2 cell lines. Complex 2 displayed photodynamic effect (400�700 nm, 10 J cm�2) giving an IC50 value of �4 μM in HeLa and MCF-7 cells, while being significantly non-toxic in the dark (IC50>100 μM). A 5-fold increase in cytotoxicity was observed in HepG2 cells (IC50�0.7 μM) for the biotin-appended complex 2. In vitro generation of reactive oxygen species (ROS) was observed from dichlorofluorescein diacetate (DCFDA) assay. The mode of cell death was apoptotic. Formation of hydroxyl radicals as the ROS was evidenced from the plasmid pUC19 DNA photo-cleavage studies. The complexes demonstrated the ability to act as targeted PDT (photodynamic therapy) drugs. © 2021 Wiley-VCH GmbH

Item Type: Journal Article
Publication: European Journal of Inorganic Chemistry
Publisher: John Wiley and Sons Inc
Additional Information: The copyright for this article belongs to John Wiley and Sons Inc
Department/Centre: Division of Biological Sciences > Biochemistry
Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited: 03 Aug 2021 10:05
Last Modified: 03 Aug 2021 10:05
URI: http://eprints.iisc.ac.in/id/eprint/69016

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