DsDNA-templated fluorescent copper nanoparticles for the detection of lipopolysaccharides

NK, R and Gorthi, SS (2021) DsDNA-templated fluorescent copper nanoparticles for the detection of lipopolysaccharides. In: Analytical Methods, 13 (2). pp. 186-191.

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Abstract

The introduction of lipopolysaccharides (LPS) or endotoxins that originate from Gram-negative bacteria into the human blood stream induces a severe immune response that can lead to septic shock, and even death. Hence, the accurate detection of LPS is of great importance in the medical and pharmaceutical sectors. This paper proposes a novel label-free fluorescence assay for the detection of LPS utilizing aptamers and the interference synthesis of dsDNA-templated copper nanoparticles. The assay can be performed at room temperature and does not require expensive reagents. The proposed assay has a limit of detection of 0.95 ng ml-1 of LPS, and the fluorescence emission from the copper nanoparticles was found to vary linearly with the concentration of LPS over a wide range (1 to 105 ng ml-1) with R2 = 0.9877. This journal is © The Royal Society of Chemistry.

Item Type:	Journal Article
Publication:	Analytical Methods
Publisher:	Royal Society of Chemistry
Additional Information:	The copyright of this article belongs to Royal Society of Chemistry
Keywords:	Bacteria; Copper; Fluorescence; Metal nanoparticles; Polysaccharides, Copper nanoparticles; Fluorescence assay; Fluorescence emission; Gram-negative bacteria; Immune response; Limit of detection; Lipopolysaccharides; Pharmaceutical sector, Synthesis (chemical)
Department/Centre:	Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	22 Feb 2021 10:47
Last Modified:	22 Feb 2021 10:47
URI:	http://eprints.iisc.ac.in/id/eprint/67938

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