Probing the Influence of ?-Sterilization on the Oxidation, Crystallization, Sliding Wear Resistance, and Cytocompatibility of Chemically Modified Graphene-Oxide-Reinforced HDPE/UHMWPE Nanocomposites and Wear Debris

Sharma, V and Bose, S and Kundu, B and Bodhak, S and Mitun, D and Balla, VK and Basu, B (2020) Probing the Influence of ?-Sterilization on the Oxidation, Crystallization, Sliding Wear Resistance, and Cytocompatibility of Chemically Modified Graphene-Oxide-Reinforced HDPE/UHMWPE Nanocomposites and Wear Debris. In: ACS Biomaterials Science and Engineering, 6 (3). pp. 1462-1475.

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Abstract

Osteolysis and aseptic loosening due to wear at the articulating interfaces of prosthetic joints are considered to be the key concerns for implant failure in load-bearing orthopedic applications. In an effort to reduce the wear and processing difficulties of ultrahigh-molecular-weight polyethylene (UHMWPE), our research group recently developed high-density polyethylene (HDPE)/UHMWPE nanocomposites with chemically modified graphene oxide (mGO). Considering the importance of sterilization, this work explores the influence of ?-ray dosage of 25 kGy on the clinically relevant performance-limiting properties of these newly developed hybrid nanocomposites in vitro. Importantly, this work also probes into the cytotoxic effects of the wear debris of different compositions and sizes on MC3T3 murine osteoblasts and human mesenchymal stem cells (hMSCs). In particular, ?-ray-sterilized 1 wt mGO-reinforced HDPE/UHMWPE nanocomposites exhibit an improvement in the oxidation index (16), free energy of immersion (-12.1 mN/m), surface polarity (5.0), and hardness (42). Consequently, such enhancements result in better tribological properties, especially coefficient of friction (+13) and wear resistance, when compared with UHMWPE. A spectrum of analyses using transmission electron microscopy (TEM) and in vitro cytocompatibility assessment demonstrate that phagocytosable (0.5-4.5 μm) sterilized 1 mGO wear particles, when present in culture media at 5 mg/mL concentration, induce neither significant reduction in MC3T3 murine osteoblast and hMSC growth nor cell morphology phenotype, during 24, 48, and 72 h of incubation. Taken together, this study suggests that ?-ray-sterilized HDPE/UHMWPE/mGO nanocomposites can be utilized as promising articulating surfaces for total joint replacements. Copyright © 2020 American Chemical Society.

Item Type:	Journal Article
Publication:	ACS Biomaterials Science and Engineering
Publisher:	American Chemical Society
Additional Information:	Copyright of this article belongs to American Chemical Society
Keywords:	Cell culture; Debris; Free energy; Friction; Graphene; Graphene oxide; High density polyethylenes; High resolution transmission electron microscopy; Joint prostheses; Morphology; Nanocomposites; Particle size analysis; Reinforcement; Stem cells; Sterilization (cleaning); Ultrahigh molecular weight polyethylenes; Wear of materials, Chemically-modified graphene; Coefficient of frictions; High density polyethylene(HDPE); Human mesenchymal stem cells (hMSCs); In-vitro; Orthopedic applications; Total joint replacement; Tribological properties, Wear resistance
Department/Centre:	Division of Chemical Sciences > Materials Research Centre Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	09 Apr 2021 11:04
Last Modified:	09 Apr 2021 11:04
URI:	http://eprints.iisc.ac.in/id/eprint/64953

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