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S-Adenosyl-L-methionine-Dependent Restriction Enzymes

Sistla, Srivani and Rao, Desirazu N (2004) S-Adenosyl-L-methionine-Dependent Restriction Enzymes. In: Critical Reviews in Biochemistry and Molecular Biology, 39 (1). pp. 1-19.

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Restriction-modification (R-M) enzymes are classified into type I, II, III, and IV, based on their recognition sequence, subunit composition, cleavage position, and cofactor requirements. While the role of S-Adenosy-L-methionine (AdoMet) as the methyl group donor in the methylation reaction is undisputed, its requirement in DNA cleavage reaction has been subject to intense study. AdoMet is a prerequisite for the DNA cleavage by most type I enzymes known so far, with the exception of R.EcoR124I. A number of new type II restriction enzymes belonging to the type IIB and IIG family were found to show AdoMet dependence for their cleavage reaction. The type III enzymes have been found to require AdoMet for their restriction function. Ado Met functions as an allosteric effector of the DNA cleavage reaction and has been shown to bring about conformational changes in the protein upon binding.

Item Type: Journal Article
Additional Information: The copyright belongs to Taylor & Francis.
Keywords: restrrction-modificafion enzymes;methyltransferase;endonuclease; AdoMet;DNA cleavage
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Anka Setty
Date Deposited: 02 May 2006
Last Modified: 27 Aug 2008 11:55
URI: http://eprints.iisc.ac.in/id/eprint/6470

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