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Phosphoprotein P of Rinderpest virus binds to plus sense leader RNA: regulation by phosphorylation

Raha, Tamal and Kaushik, Rajnish and Shaila, MS (2004) Phosphoprotein P of Rinderpest virus binds to plus sense leader RNA: regulation by phosphorylation. In: Virus Research, 104 (2). pp. 191-200.

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The negative sense genome RNA of Rinderpest virus, a Paramyxoviridae, is encapsidated with the nucleocapsid protein N and serves as a template for the viral RNA dependent RNA polymerase for transcription and replication. The viral RNA polymerase consists of the large protein L and the phosphoprotein P functioning as the P–L complex. We provide in this report, evidences for specific binding of P protein of Rinderpest virus to the plus sense leader RNA depending on its phosphorylation status. We have also demonstrated that P protein is released from the le RNA:P protein complex upon phosphorylation in vitro. Finally, we have identified that the C-terminal 358–389 amino acid residues of P protein is involved in le RNA binding. The leader RNA binding may signify a hitherto unidentified role for P protein in the viral RNA synthesis. Moreover, our results indicate a possible role for P protein in the transcription-replication switch through leader RNA binding.

Item Type: Journal Article
Additional Information: The copyright belongs to Elsevier.
Keywords: Rinderpest virus;Phosphoprotein;Leader RNA;Replication and transcription
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Depositing User: Anka Setty
Date Deposited: 24 Apr 2006
Last Modified: 19 Sep 2010 04:25
URI: http://eprints.iisc.ac.in/id/eprint/6439

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