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Direct Detection of Bacteria Using Positively Charged Ag/Au Bimetallic Nanoparticles: A Label-free Surface-Enhanced Raman Scattering Study Coupled with Multivariate Analysis

Prakash, O and Sil, S and Verma, T and Umapathy, S (2019) Direct Detection of Bacteria Using Positively Charged Ag/Au Bimetallic Nanoparticles: A Label-free Surface-Enhanced Raman Scattering Study Coupled with Multivariate Analysis. In: Journal of Physical Chemistry C, 124 (1). pp. 861-869.

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Official URL: https://dx.doi.org/10.1021/acs.jpcc.9b09311

Abstract

Rapid detection and discrimination of pathogenic bacteria for food safety, environmental pollution, medical diagnoses, and chemical and biological threats remains a considerable challenge. In the present work, we demonstrate positively charged Ag/Au bimetallic nanoparticles (Ag/Au bmNPs) as a potential surface-enhanced Raman scattering (SERS) substrate for label-free detection and discrimination of three bacteria, viz., Escherichia coli, Salmonella typhimurium, and Bacillus subtilis with excellent reproducibility. The approach relies on a priori synthesis of Ag/Au bmNPs and subsequent SERS studies on bacteria. The positive surface charge on Ag/Au bmNPs offers significant advantages of short acquisition time at very low power, high sensitivity, and a simple operating procedure without the need of very specific procedures or protocols used to capture the bacteria. The reproducible and specific intrinsic fingerprint of the cell wall and intracellular components of three bacteria obtained by label-free SERS enables precise discrimination and classification of three bacteria using multivariate analyses such as principal component analysis and canonical discriminant analysis.

Item Type: Journal Article
Publication: Journal of Physical Chemistry C
Publisher: American Chemical Society
Additional Information: Copyright of this article belongs to American Chemical Society
Keywords: Bacteriology; Chemical detection; Diagnosis; Discriminant analysis; Escherichia coli; Fault tolerant computer systems; Food safety; Multivariant analysis; Nanoparticles; Natural language processing systems; Principal component analysis; Raman scattering; Salmonella; Substrates, Bimetallic nanoparticles; Canonical discriminant analysis; Chemical and biologicals; Environmental pollutions; Intracellular components; Multi variate analysis; Positive surface charge; Salmonella typhimurium, Surface scattering
Department/Centre: Division of Chemical Sciences > Inorganic & Physical Chemistry
Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering
Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited: 24 Jan 2020 10:12
Last Modified: 24 Jan 2020 10:12
URI: http://eprints.iisc.ac.in/id/eprint/64359

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