UvrA and UvrC subunits of the Mycobacterium tuberculosis UvrABC excinuclease interact independently of UvrB and DNA

Thakur, Manoj and Badugu, Sugith and Muniyappa, Kalappa (Kalappagowda) (2019) UvrA and UvrC subunits of the Mycobacterium tuberculosis UvrABC excinuclease interact independently of UvrB and DNA. In: FEBS LETTERS .

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Abstract

The UvrABC excinuclease plays a vital role in bacterial nucleotide excision repair. While UvrA and UvrB subunits associate to form a UvrA(2)B(2) complex, interaction between UvrA and UvrC has not been demonstrated or quantified in any bacterial species. Here, using Mycobacterium tuberculosis UvrA (MtUvrA), UvrB (MtUvrB) and UvrC (MtUvrC) subunits, we show that MtUvrA binds to MtUvrB and equally well to MtUvrC with submicromolar affinity. Furthermore, MtUvrA forms a complex with MtUvrC both in vivo and in vitro, independently of DNA and UvrB. Collectively, these findings reveal new insights into the pairwise relationships between the subunits of the UvrABC incision complex.

Item Type:	Journal Article
Publication:	FEBS LETTERS
Publisher:	WILEY
Additional Information:	Copyright of this article belongs to WILEY
Keywords:	M; tuberculosis; nucleotide excision repair; protein-protein interaction; UvrABC excinuclease; UvrA-UvrC complex
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	17 Dec 2019 09:19
Last Modified:	17 Dec 2019 09:19
URI:	http://eprints.iisc.ac.in/id/eprint/64042

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