ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Investigation of anti-cancer and migrastatic properties of novel curcumin derivatives on breast and ovarian cancer cell lines

Koroth, Jinsha and Nirgude, Snehal and Tiwari, Shweta and Gopalakrishnan, Vidya and Mahadeva, Raghunandan and Kumar, Sujeet and Karki, Subhas S and Choudhary, Bibha (2019) Investigation of anti-cancer and migrastatic properties of novel curcumin derivatives on breast and ovarian cancer cell lines. In: BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, 19 (1).

[img] Microsoft Word
12906_2019_2685_MOESM1_ESM .doc - Published Supplemental Material

Download (1MB)
[img]
Preview
PDF
bmc_com_alt_med_19-1_2019.pdf - Published Version

Download (2MB) | Preview
Official URL: http://dx.doi.org/10.1186/s12906-019-2685-3

Abstract

Background Curcumin is known for its multitude of medicinal properties, including anti-cancer and migrastatic activity. Efforts to overcome poor bioavailability, stability, and side effects associated with the higher dose of curcumin has led to the development of newer derivatives of curcumin. Thus, the focus of this study is to screen novel curcumin derivatives, namely ST03 and ST08, which have not been reported before, for their cytotoxicity and migrastatic property on cancer cells. Methods Anti-cancer activity of ST03 and ST08 was carried out using standard cytotoxicity assays viz., LDH, MTT, and Trypan blue on both solid and liquid cancer types. Flow cytometric assays and western blotting was used to investigate the cell death mechanisms. Transwell migration assay was carried out to check for migrastatic properties of the compounds. Results Both the compounds, ST03 and ST08, showed similar to 100 fold higher potency on liquid and solid tumour cell lines compared to its parent compound curcumin. They induced cytotoxicity by activating the intrinsic pathway of apoptosis in the breast (MDA-MB-231) and ovarian cancer cell lines (PA-1) bearing metastatic and stem cell properties, respectively. Moreover, ST08 also showed inhibition on breast cancer cell migration by inhibiting MMP1 (matrix metalloproteinase 1). Conclusion Both ST03 and ST08 exhibit anti-cancer activity at nanomolar concentration. They induce cell death by activating the intrinsic pathway of apoptosis. Also, they inhibit migration of the cancer cells by inhibiting MMP1 in breast cancer cells.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to BMC
Keywords: Cancer therapy; Curcumin derivatives; PA1; MDA-MB-231; Anti-cancer; Migrastatic
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Id for Latest eprints
Date Deposited: 27 Nov 2019 10:23
Last Modified: 27 Nov 2019 10:23
URI: http://eprints.iisc.ac.in/id/eprint/63818

Actions (login required)

View Item View Item