Paederia foetida induces anticancer activity by modulating chromatin modification enzymes and altering pro-inflammatory cytokine gene expression in human prostate cancer cells

Pradhan, Nibedita and Parbin, Sabnam and Kausar, Chahat and Kar, Swayamsiddha and Mawatwal, Shradha and Das, Laxmidhar and Deb, Moonmoon and Sengupta, Dipta and Dhiman, Rohan and Patra, Samir Kumar (2019) Paederia foetida induces anticancer activity by modulating chromatin modification enzymes and altering pro-inflammatory cytokine gene expression in human prostate cancer cells. In: FOOD AND CHEMICAL TOXICOLOGY, 130 . pp. 161-173.

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Abstract

Aberrant epigenetic modifications are responsible for tumor development and cancer progression; however, readily reversible. Bioactive molecules from diets are promising to cure cancer by modulating epigenetic marks and changing immune response. These compounds specifically target the activity of DNMTs and HDACs to cure various human cancers. In view of this, we investigated the anticancer and epigenetic regulatory activities of an edible-plant Paederia foetida. The efficacy of methanolic extract of P. foetida leaves (MEPL) was tested for the modulation of epigenetic factors in gene silencing, i.e. DNMT and HDAC and expression pattern of certain tumor suppressor genes. After treatment of prostate cancer cells (PC-3 and DU-145) with MEPL, lupeol and beta-sitosterol; induction of apoptosis, decrease in cellular-viability and inhibition of cellular-migration were noticed. Simultaneously there was inhibition of DNMT1, HDACs and pro-inflammatory, IL-6, IL1-beta, TNF-alpha and anti-inflammatory, IL-10 genes in cancer and THP1 cell lines. The DNMT1 protein content, enzyme activity and Bcl2 expression decreased significantly; however, expression of E-cadherin (CDH1) and pro-apoptotic gene Bax increased significantly after the treatment of cells with drugs. We conclude plant-derived compounds can be considered to target epigenetic machineries involved with malignant transformation and can open new avenues for cancer therapeutics provoking immune response.

Item Type:	Journal Article
Publication:	FOOD AND CHEMICAL TOXICOLOGY
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Additional Information:	copyright for this article belongs to PERGAMON-ELSEVIER SCIENCE LTD
Keywords:	Paederia foetida; prostate cancer; Epigenetics and DNA methyltransferases; E-cadherin; Pro-inflammatory cytokines; Inflammation & immunity
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	17 Sep 2019 05:50
Last Modified:	17 Sep 2019 05:50
URI:	http://eprints.iisc.ac.in/id/eprint/63213

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