Harris, Clair and Cloutier, Marissa and Trotter, Megan and Hinten, Michael and Gayen, Srimonta and Du, Zhenhai and Xie, Wei and Kalantry, Sundeep (2019) Conversion of random X-inactivation to imprinted X-inactivation by maternal PRC2. In: ELIFE, 8 .
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Abstract
Imprinted X-inactivation silences genes exclusively on the paternally-inherited X-chromosome and is a paradigm of transgenerational epigenetic inheritance in mammals. Here, we test the role of maternal vs. zygotic Polycomb repressive complex 2 (PRC2) protein EED in orchestrating imprinted X-inactivation in mouse embryos. In maternal-null (Eed(m-/-)) but not zygotic null (Eed(-/-)) early embryos, the maternal X-chromosome ectopically induced Xist and underwent inactivation. Eed(m-/-) females subsequently stochastically silenced Xist from one of the two X-chromosomes and displayed random X-inactivation. This effect was exacerbated in embryos lacking both maternal and zygotic EED (Eed(mz-/-)), suggesting that zygotic EED can also contribute to the onset of imprinted X-inactivation. Xist expression dynamics in Eed(m-/-) embryos resemble that of early human embryos, which lack oocyte-derived maternal PRC2 and only undergo random X-inactivation. Thus, expression of PRC2 in the oocyte and transmission of the gene products to the embryo may dictate the occurrence of imprinted X-inactivation in mammals.
| Item Type: | Journal Article |
|---|---|
| Publication: | ELIFE |
| Publisher: | ELIFE SCIENCES PUBLICATIONS LTD |
| Additional Information: | copyright for this article belongs to the Authors |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics |
| Date Deposited: | 11 Jul 2019 07:28 |
| Last Modified: | 11 Jul 2019 07:28 |
| URI: | http://eprints.iisc.ac.in/id/eprint/62974 |
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