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On-Field Detection of Helicoverpa armigera Nuclear Polyhedrosis Virus Using Luminescent Amphiphilic Probe: Screening of Agricultural Crops and Commercial Formulations

Dey, Nilanjan and Bhagat, Deepa and Bhattacharya, Santanu (2019) On-Field Detection of Helicoverpa armigera Nuclear Polyhedrosis Virus Using Luminescent Amphiphilic Probe: Screening of Agricultural Crops and Commercial Formulations. In: ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 7 (8). pp. 7667-7675.

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Official URL: https://doi.org/10.1021/acssuschemeng.8b06152

Abstract

An easy to synthesize luminescent, amphiphilic probe has been designed for the first time in optical sensing of a biopesticide, Helicoverpa armigera nuclear polyhedrosis virus (HaNPV). The compound showed formation of a pH-sensitive, thermoreversible nanoaggregate in the aqueous medium. The addition of HaNPV resulted in the rapid change in emission color from blue to cyan at pH 7.4. Till date, no optical assay is known in the literature, which can achieve fast, on-field detection of HaNPV as low as similar to 10(3) POBs/mL. Most importantly, because of naked-eye response, this method does not need the involvement of trained personnel or any sophisticated visualizing instrument. In addition, the distinct optical response also allows us to distinguish the freshly prepared pesticide solution from its storage-old analogue. The quantification of HaNPV is achieved in a wide range of agricultural crop extracts, known to be infected by H. armigera. Inexpensive reusable paper strips are developed for on-location detection purposes. Moreover, the presence of residual HaNPV can also be traced on leaf surfaces. Thus, studies of such kind will be beneficial for quality verification of HaNPV and will surely add a new dimension to the better management of H. armigera and minimize the extent of crop loss.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to ACS SUSTAINABLE CHEMISTRY & ENGINEERING
Keywords: Biopesticide; Fluorimetric sensing; Commercial formulations; Agricultural crops; Paper discs
Department/Centre: Division of Chemical Sciences > Organic Chemistry
Depositing User: Id for Latest eprints
Date Deposited: 23 May 2019 05:58
Last Modified: 23 May 2019 05:58
URI: http://eprints.iisc.ac.in/id/eprint/62628

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