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Sequential backbone resonance assignment of AT-rich interaction domain of human BAF200

Bastiray, Abhishek and Giri, Malyasree and Singh, Mahavir (2019) Sequential backbone resonance assignment of AT-rich interaction domain of human BAF200. In: BIOMOLECULAR NMR ASSIGNMENTS, 13 (1). pp. 115-119.

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Official URL: https://dx.doi.org/10.1007/s12104-018-9862-z


BAF200 is a subunit of PBAF chromatin remodeling complex that contains an N-terminal AT-rich interaction domain (ARID). ARID domain in general has been shown to bind to the AT-rich DNA sequences. The human BAF200 ARID (similar to 110 residues) has the potential to bind the DNA sequences with high affinity, however, the structure and the exact contribution of hBAF200 ARID in PBAF functions as well its DNA binding specificities have not been established. In this study, we have expressed and purified the hBAF200 ARID for NMR studies. We report the complete backbone H-1, C-13, and N-15 chemical shift assignment and secondary structure of hBAF200 ARID domain.

Item Type: Journal Article
Additional Information: Copyright of this articles belongs to BIOMOLECULAR NMR ASSIGNMENTS
Keywords: SWI; SNF complex; hBAF200; ARID; NMR; Backbone assignment
Department/Centre: Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Depositing User: Id for Latest eprints
Date Deposited: 13 May 2019 12:20
Last Modified: 13 May 2019 12:20
URI: http://eprints.iisc.ac.in/id/eprint/62325

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