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Targeted Proteomics Comes to the Benchside and the Bedside: Is it Ready for Us?

Arora, Anjali and Somasundaram, Kumaravel (2019) Targeted Proteomics Comes to the Benchside and the Bedside: Is it Ready for Us? In: BIOESSAYS, 41 (2).

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Official URL: http://dx.doi.org/ 10.1002/bies.201800042

Abstract

While mass spectrometry (MS)-based quantification of small molecules has been successfully used for decades, targeted MS has only recently been used by the proteomics community to investigate clinical questions such as biomarker verification and validation. Targeted MS holds the promise of a paradigm shift in the quantitative determination of proteins. Nevertheless, targeted quantitative proteomics requires improvisation in making sample processing, instruments, and data analysis more accessible. In the backdrop of the genomic era reaching its zenith, certain questions arise: is the proteomic era about to come? If we are at the beginning of a new future for protein quantification, are we prepared to incorporate targeted proteomics at the benchside for basic research and at the bedside for the good of patients? Here, an overview of the knowledge required to perform targeted proteomics as well as its applications is provided. A special emphasis is placed on upcoming areas such as peptidomics, proteoform research, and mass spectrometry imaging, where the utilization of targeted proteomics is expected to bring forth new avenues. The limitations associated with the acceptance of this technique for mainstream usage are also highlighted. Also see the video abstract here https://youtu.be/mieB47B8gZw.

Item Type: Journal Article
Additional Information: Copyright for this article belongs to WILEY
Keywords: mass spectrometry; MRM; PRM; SRM; targeted proteomics
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Depositing User: Id for Latest eprints
Date Deposited: 26 Mar 2019 07:49
Last Modified: 26 Mar 2019 07:49
URI: http://eprints.iisc.ac.in/id/eprint/61943

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