Functionalized Two-Dimensional MoS2 with Tunable Charges for Selective Enzyme Inhibition

Karunakaran, Subbaraj and Pandit, Subhendu and De, Mrinmoy (2018) Functionalized Two-Dimensional MoS2 with Tunable Charges for Selective Enzyme Inhibition. In: ACS OMEGA, 3 (12). pp. 17532-17539.

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Abstract

Recent advances in the synthesis and functionalization of two-dimensional (2D) nanomaterials have allowed us to explore their interaction with biological systems. 2D nanomaterials, owing to their unique layered structure and a high surface-to-volume ratio, are very promising systems for various biological applications. Transition metal dichalcogenides (TMDs) are a unique class of 2D nanomaterials known for their easy surface functionalization using thiol ligands. Interactions of functionalized MoS2, a TMD, with different proteins are worth exploring as that might give us an overall insight about the interaction of these materials with bio-macromolecules. Here, we have chemically exfoliated MoS2 and functionalized it with surface groups having different charges (negative, neutral, positive, and zwitterionic). Interaction of these functionalized MoS2 with two model proteins, chymotrypsin (ChT) and beta-galactosidase (beta-gal), were explored. Positively charged MoS2 inhibited the enzymatic activity of beta-gal, whereas the activity of ChT was inhibited by negatively charged MoS2. Polyethylene glycol- functionalized neutral MoS2 did not affect any of the proteins, but the zwitterionic ligand-functionalized MoS2 inhibits ChT and causes hyperactivity in beta-gal. The nature of the inhibition was found to be noncompetitive. We also studied the changes in the secondary structure of proteins upon inhibition to assess their biocompatibility. In brief, we have explored interactions between differently functionalized MoS2 nanomaterials and two model proteins, ChT and beta-gal, as a proof of concept study toward the future development of 2D material-based enzyme inhibitors and for their other biological applications.

Item Type:	Journal Article
Publication:	ACS OMEGA
Publisher:	AMER CHEMICAL SOC
Additional Information:	Copyright of this article belongs to AMER CHEMICAL SOC
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	28 Jan 2019 09:33
Last Modified:	28 Jan 2019 09:33
URI:	http://eprints.iisc.ac.in/id/eprint/61495

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