ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

EpsN from Bacillus subtilis 168 has UDP-2,6-dideoxy 2-acetamido 4-keto glucose aminotransferase activity in vitro

Kaundinya, Chinmayi R and Savithri, Handanahal S and Rao, Krishnamurthy K and Balaji, Petety V (2018) EpsN from Bacillus subtilis 168 has UDP-2,6-dideoxy 2-acetamido 4-keto glucose aminotransferase activity in vitro. In: GLYCOBIOLOGY, 28 (10). pp. 802-812.

[img] PDF
Geo_28_10_802_2018.pdf - Published Version
Restricted to Registered users only

Download (1MB) | Request a copy
Official URL: https://doi.org/ 10.1093/glycob/cwy063

Abstract

The gene epsN of Bacillus subtilis 168 was cloned and overexpressed in Escherichia coli. Purified recombinant EpsN is shown to be a pyridoxal 5'-phosphate (PLP)-dependent aminotransferase by absorption spectroscopy, L-cycloserine inhibition and reverse phase HPLC studies. EpsN catalyzes the conversion of UDP-2,6-dideoxy 2-acetamido 4-keto glucose to UDP-2,6-dideoxy 2-acetamido 4-amino glucose. Lys190 was found by sequence comparison and site-directed mutagenesis to form Schiff base with PLP. Mutagenesis studies showed that, in addition to Lys190, Ser185, Glu164, Gly58 and Thr59 are essential for aminotransferase activity.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to OXFORD UNIV PRESS INC
Keywords: deoxyaminosugars; eps operon; nucleotide sugar pyridoxal 5 `-phosphate dependent aminotransferase (SATs); N,N `-diacetylbacillosamine biosynthetic pathway enzyme
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Francis Jayakanth
Date Deposited: 24 Jan 2019 10:15
Last Modified: 24 Jan 2019 10:15
URI: http://eprints.iisc.ac.in/id/eprint/61404

Actions (login required)

View Item View Item