Inhaled bacteriophage-loaded polymeric microparticles ameliorate acute lung infections

Agarwal, Rachit and Johnson, Christopher T and Imhoff, Barry R and Donlan, Rodney M and McCarty, Nael A and Garcia, Andres J (2018) Inhaled bacteriophage-loaded polymeric microparticles ameliorate acute lung infections. In: NATURE BIOMEDICAL ENGINEERING, 2 (11). pp. 841-849.

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Abstract

Lung infections associated with pneumonia, or cystic fibrosis caused by Pseudomonas aeruginosa or other bacteria, result in significant morbidity and mortality, in part owing to the development of multidrug resistance, also against last-resort antibiotics. Lytic bacteriophages (that is, viruses that specifically kill bacteria) can reduce lung-associated infections, yet their clinical use is hindered by difficulties in delivering active phages to the deep lung. Here, we show that phage-loaded polymeric microparticles deposit throughout the lung via dry powder inhalation and that they deliver active phages. Phage-loaded microparticles effectively reduced P. aeruginosa infections and the associated inflammation in wild-type and cystic fibrosis trans-membrane-conductance-regulator knockout mice, and rescued the mice from pneumonia-associated death. These polymeric microparticles might constitute a clinically translatable therapy for eradicating hospital-acquired lung infections and infections associated with cystic fibrosis.

Item Type:	Journal Article
Publication:	NATURE BIOMEDICAL ENGINEERING
Publisher:	NATURE PUBLISHING GROUP
Additional Information:	Copy right for this article belong to NATURE PUBLISHING GROUP
Department/Centre:	Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering
Date Deposited:	28 Nov 2018 15:13
Last Modified:	28 Nov 2018 15:13
URI:	http://eprints.iisc.ac.in/id/eprint/61175

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