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A secreted Heat shock protein 90 of Trichomonas vaginalis

Singh, Meetali and Beri, Divya and Nageshan, Rishi Kumar and Chavaan, Leena and Gadara, Darshak and Poojary, Mukta and Subramaniam, Suraj and Tatu, Utpal (2018) A secreted Heat shock protein 90 of Trichomonas vaginalis. In: PLOS NEGLECTED TROPICAL DISEASES, 12 (5).

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Official URL: https://dx.doi.org/10.1371/journal.pntd.0006493

Abstract

Trichomonas vaginalis is a causative agent of Trichomoniasis, a leading non-viral sexually transmitted disease worldwide. In the current study, we show Heat shock protein 90 is essential for its growth. Upon genomic analysis of the parasite, it was found to possess seven ORFs which could potentially encode Hsp90 isoforms. We identified a cytosolic Hsp90 homolog, four homologs which can align to truncated cytosolic Hsp90 gene products along with two Grp94 homologs (ER isoform of Hsp90). However, both Grp94 orthologs lacked an ER retention motif. In cancer cells, it is very well established that Hsp90 is secreted and regulates key clients involved in metastases, migration, and invasion. Since Trichomonas Grp94 lacks ER retention motif, we examined the possibility of its secretion. By using cell biology and biochemical approaches we show that the Grp94 isoform of Hsp90 is secreted by the parasite by the classical ER-Golgi pathway. This is the first report of a genome encoded secreted Hsp90 in a clinically important parasitic protozoan.

Item Type: Journal Article
Additional Information: Copyright of this article belong to PUBLIC LIBRARY SCIENCE, 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Id for Latest eprints
Date Deposited: 26 Jul 2018 15:08
Last Modified: 26 Jul 2018 15:08
URI: http://eprints.iisc.ac.in/id/eprint/60305

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