Manganese-Based Nanozymes: Multienzyme Redox Activity and Effect on the Nitric Oxide Produced by Endothelial Nitric Oxide Synthase

Singh, Namrata and Geethika, Motika and Eswarappa, Sandeep M and Mugesh, Govindasamy (2018) Manganese-Based Nanozymes: Multienzyme Redox Activity and Effect on the Nitric Oxide Produced by Endothelial Nitric Oxide Synthase. In: CHEMISTRY-A EUROPEAN JOURNAL, 24 (33). pp. 8393-8403.

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Abstract

Nanomaterials having enzyme-like activity (nanozymes) make them suitable candidates for various biomedical applications. In this study, we demonstrate the morphology-dependent enzyme mimetic activity of Mn3O4 nanoparticles. It is found that Mn3O4 nanoparticles mimic the functions of all three cellular antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). Interestingly, the nanozyme activity of Mn3O4 depends on various factors including size, morphology, surface area, and the redox properties of the metal ions. The Mn3O4 nanoflowers exhibited remarkably high activity in all three enzyme systems and the order of multienzyme activity of different morphologies was: flowers >> flakes > hexagonal plates approximate to polyhedrons approximate to cubes. Interestingly, all five nanoforms are taken up by the mammalian cells and were found to be biocompatible, with very low cytotoxicity. The activity of the most active nanoflowers was studied in primary human umbilical vein endothelial cells (HUVEC) and human pulmonary microvascular endothelial cells (hPMEC) and it was found that Mn3O4 does not reduce the level of nitric oxide (NO). This is in contrast to the effect of some of the Mn-porphyrin-based SOD mimetics, which are known to scavenge NO in endothelial cells.

Item Type:	Journal Article
Publication:	CHEMISTRY-A EUROPEAN JOURNAL
Publisher:	WILEY-V C H VERLAG GMBH, POSTFACH 101161, 69451 WEINHEIM, GERMANY
Additional Information:	Copyright of this article belong to WILEY-V C H VERLAG GMBH, POSTFACH 101161, 69451 WEINHEIM, GERMANY
Department/Centre:	Division of Biological Sciences > Biochemistry Division of Chemical Sciences > Inorganic & Physical Chemistry Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited:	16 Jul 2018 16:19
Last Modified:	16 Jul 2018 16:19
URI:	http://eprints.iisc.ac.in/id/eprint/60189

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