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Eu/Tb luminescence for alkaline phosphatase and beta-galactosidase assay in hydrogels and on paper devices

Gorai, Tumpa and Maitra, Uday (2018) Eu/Tb luminescence for alkaline phosphatase and beta-galactosidase assay in hydrogels and on paper devices. In: JOURNAL OF MATERIALS CHEMISTRY B, 6 (14). pp. 2143-2150.

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Official URL: http://dx.doi.org/ 10.1039/c7tb02657a

Abstract

Simple technologies for efficient detection of important (bio) molecules are always in great demand. We now report the detection and assay of two biologically important enzymes, alkaline phosphatase and beta-galactosidase, in Eu- or Tb-based cholate hydrogels, respectively, and on filter paper discs coated with such hydrogels. Pro-sensitizers derived from 1-hydroxypyrene and 2,3-dihydroxynaphthalene were incorporated into Eu or Tb cholate hydrogels, respectively. Upon enzyme action, these artificial substrates liberate free sensitizers both in the gel and on gel-coated discs, resulting in turn-on luminescence, red/magenta for Eu, and green for Tb. The detection of enzymes was also demonstrated in natural/biological samples using low-cost systems.

Item Type: Journal Article
Additional Information: Copy right for this article belong to ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Department/Centre: Division of Chemical Sciences > Organic Chemistry
Depositing User: Id for Latest eprints
Date Deposited: 17 May 2018 18:27
Last Modified: 17 May 2018 18:27
URI: http://eprints.iisc.ac.in/id/eprint/59879

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