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Phase modulated 2D HSQC-TOCSY for unambiguous assignment of overlapping spin systems

Singh, Amrinder and Dubey, Abhinav and Adiga, Satish Kumar and Atreya, Hanudatta S (2018) Phase modulated 2D HSQC-TOCSY for unambiguous assignment of overlapping spin systems. In: JOURNAL OF MAGNETIC RESONANCE, 286 . pp. 10-16.

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Official URL: http://dx.doi.org/10.1016/j.jmr.2017.11.005


We present a new method that allows one to unambiguously resolve overlapping spin systems often encountered in biomolecular systems such as peptides and proteins or in samples containing a mixture of different molecules such as in metabolomics. We address this problem using the recently proposed phase modulation approach. By evolving the H-1 chemical shifts in a conventional two dimensional (2D) HSQC-TOCSY experiment for a fixed delay period, the phase/intensity of set of cross peaks belonging to one spin system are modulated differentially relative to those of its overlapping counterpart, resulting in their discrimination and recognition. The method thus accelerates the process of identification and resonance assignment of individual compounds in complex mixtures. This approach facilitated the assignment of molecules in the embryo culture medium used in human assisted reproductive technology. (C) 2017 Elsevier Inc. All rights reserved.

Item Type: Journal Article
Additional Information: Copy right for this article belong to the ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
Department/Centre: Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Depositing User: Id for Latest eprints
Date Deposited: 02 Mar 2018 14:55
Last Modified: 18 Feb 2019 10:20
URI: http://eprints.iisc.ac.in/id/eprint/59057

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