Creatinine-Iron Complex and Its Use in Electrochemical Measurement of Urine Creatinine

Kumar, Vinay and Hebbar, Suraj and Kalam, Rahila and Panwar, Sachin and Prasad, Sujay and Srikanta, SS and Krishnaswamy, PR and Bhat, Navakanta (2018) Creatinine-Iron Complex and Its Use in Electrochemical Measurement of Urine Creatinine. In: IEEE SENSORS JOURNAL, 18 (2). pp. 830-836.

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Abstract

A non-enzymatic electrochemical technique for creatinine sensing is presented, exploiting iron binding property of creatinine. Disposable carbon printed electrodes layered with FeCl3 coated cotton fiber membranes are used to sense creatinine from 10 to 245 mg/dl, on clinical urine samples. The energy-dispersive X-ray spectroscopy analysis confirms the presence of Fe(III) dry chemistry on cotton membrane. Creatinine binding with Fe(III) is verified with UV analysis, with a corresponding decrease in Fe(III) reduction current in cyclic voltammetry. The disposable test strips are interfaced with multi-potentiostat point of care (POC) hand-held device, working in amperometry mode. The results obtained on POC biosensors demonstrate good correlation (R-2 = 0.91) with Jaffe method laboratory gold standard. The intra-assay variability is less than 7.1%. The statistical bias as revealed from the Bland-Altman analysis indicates that the POC results are within 95% confidence interval. This POC device does not require any sample preparation step and provides sample to result in less than a minute. FeCl3 sensing chemistry is robust against urine albumin interference, which is especially significant for accurate estimation of albumin to creatinine ratio. The non-enzymatic nature of disposable test strips results in highly stable and robust operation of the POC device over a large range of temperature variations.

Item Type:	Journal Article
Publication:	IEEE SENSORS JOURNAL
Publisher:	10.1109/JSEN.2017.2777913
Additional Information:	Copy right for this article belongs to the IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC, 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA
Department/Centre:	Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited:	20 Jan 2018 06:54
Last Modified:	20 Jan 2018 06:54
URI:	http://eprints.iisc.ac.in/id/eprint/58805

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