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Anti-inflammatory effect of a novel formulation of coconut inflorescence sap against ox-LDL induced inflammatory responses in human peripheral blood mononuclear cells by modulating TLR-NF-kappa B signaling pathway

Ratheesh, M and Svenia, Jose P and Asha, S and Sandya, S and Girishkumar, B and Krishnakumar, IM (2017) Anti-inflammatory effect of a novel formulation of coconut inflorescence sap against ox-LDL induced inflammatory responses in human peripheral blood mononuclear cells by modulating TLR-NF-kappa B signaling pathway. In: TOXICOLOGY MECHANISMS AND METHODS, 27 (8). pp. 615-621.

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Official URL: http://dx.doi.org/10.1080/15376516.2017.1344339

Abstract

Oxidized low density lipoprotein (ox LDL) induced inflammatory response was reported to play an important role in the pathogenesis of atherosclerosis. The purpose of this study was to explore the anti-inflammatory effect of a novel formulation of coconut inflorescence sap (CSP); COCOZENTM against ox-LDL induced inflammatory responses in human peripheral blood mononuclear cells (hPBMCs). The hPBMCs were isolated from healthy human volunteers and cultured in collagen coated plates at 37 degrees C. The cells were grouped as Group I (Control), Group II (ox-LDL treated) and Group III (ox-LDLthornCSP treated). Further analysis of inflammatory markers, reactive oxygen species, mRNA and protein expression levels indicated increased expressions of TLR-4, TNF-alpha, IL-6 and VCAM-1 in ox-LDL treated group along with the nuclear translocation of NF-kappa B. Other inflammatory markers such as LOX, PGE2, NO, total COX and lipid peroxidation level were also found to be significantly (p<. 05) increased upon Ox-LDL treatment. The treatment with CSP on the other hand was found to down regulate and reverse the ox-LDL-induced alterations indicating its potential anti-inflammatory effect on hPBMCs via TLR-NF-kappa B signaling pathway.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the TAYLOR & FRANCIS LTD, 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND
Department/Centre: Division of Chemical Sciences > Inorganic & Physical Chemistry
Depositing User: review EPrints Reviewer
Date Deposited: 12 Jan 2018 06:47
Last Modified: 12 Jan 2018 06:47
URI: http://eprints.iisc.ac.in/id/eprint/58793

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