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SOFAST-HMQC-an efficient tool for metabolomics

Ghosh, Soumita and Sengupta, Arjun and Chandra, Kousik (2017) SOFAST-HMQC-an efficient tool for metabolomics. In: ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 409 (29). pp. 6731-6738.

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Official URL: http://doi.org/10.1007/s00216-017-0676-0


Nuclear magnetic resonance (NMR)-based metabolomics relies mostly on 1D NMR; however, the technique is limited by overlap of the signals from the metabolites. In order to circumvent this problem, 2D H-1-C-13 correlation spectroscopy techniques are often used. However owing to poorer natural abundance and gyromagnetic ratio of C-13, the acquisition time for 2D H-1-C-13 heteronuclear single quantum coherence spectroscopy (HSQC) is long. This makes it almost impossible to be used in high throughput study. We have reported the application of selective optimized flip angle short transient (SOFAST) technique coupled to heteronuclear multiple quantum correlation (HMQC) along with nonlinear sampling (NUS) in urine and serum samples. This technique takes sevenfold less experimental time than the conventional H-1-C-13 HSQC experiment with retention of almost all molecular information. Hence, this can be used for high throughput study.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the SPRINGER HEIDELBERG, TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY
Department/Centre: Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Depositing User: Id for Latest eprints
Date Deposited: 24 Nov 2017 10:12
Last Modified: 24 Nov 2017 10:12
URI: http://eprints.iisc.ac.in/id/eprint/58304

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