Interplay between PTB and miR-1285 at the p53 3 ` UTR modulates the levels of p53 and its isoform Delta 40p53 alpha

Katoch, Aanchal and George, Biju and Iyyappan, Amrutha and Khan, Debjit and Das, Saumitra (2017) Interplay between PTB and miR-1285 at the p53 3 ` UTR modulates the levels of p53 and its isoform Delta 40p53 alpha. In: NUCLEIC ACIDS RESEARCH, 45 (17). pp. 10206-10217.

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Abstract

p53 and its translational isoform Delta 40p53 are involved in many important cellular functions like cell cycle, cell proliferation, differentiation and metabolism. Expression of both the isoforms can be regulated at different steps. In this study, we explored the role of 3 ` UTR in regulating the expression of these two translational isoforms. We report that the trans acting factor, Polypyrimidine Tract Binding protein (PTB), also interacts specifically with 3 ` UTR of p53 mRNA and positively regulates expression of p53 isoforms. Our results suggest that there is interplay between miRNAs and PTB at the 3 ` UTR under normal and stress conditions like DNA damage. Interestingly, PTB showed some overlapping binding regions in the p53 3 ` UTR with miR-1285. In fact, knockdown of miR-1285 as well as expression of p53 3 ` UTR with mutated miR-1285 binding sites resulted in enhanced association of PTB with the 3 ` UTR, which provides mechanistic insights of this interplay. Taken together, the results provide a plausible molecular basis of how the interplay between miRNAs and the PTB protein at the 3 ` UTR can play pivotal role in fine tuning the expression of the two p53 isoforms.

Item Type:	Journal Article
Publication:	NUCLEIC ACIDS RESEARCH
Additional Information:	Copy right for this article belongs to the OXFORD UNIV PRESS, GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	21 Oct 2017 06:18
Last Modified:	21 Oct 2017 06:18
URI:	http://eprints.iisc.ac.in/id/eprint/58049

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