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Flexible, Label-Free DNA Sensor Using Platinum Oxide as the Sensing Element

Basu, Nivedita and Konduri, Anil Krishna and Basu, Palash Kumar and Keshavan, Sandeep and Varma, Manoj M and Bhat, Navakanta (2017) Flexible, Label-Free DNA Sensor Using Platinum Oxide as the Sensing Element. In: IEEE SENSORS JOURNAL, 17 (19). pp. 6140-6147.

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Official URL: http://doi.org/10.1109/JSEN.2017.2738567

Abstract

Platinum oxide thin film (100 nm) deposited using an optimized reactive ion sputtering process revealed p-type semiconducting behavior with a band-gap of 1.5 eV, resistivity of 0.16 Omega-m, and activation energy of 0.22 eV. XPS spectra indicated the presence of PtO phase (32%) along with PtO2\textbackslash phase (68%). The XRD spectra indicated the formation of alpha-PtO2 phase. arrays of simple, two terminal sensors were fabricated on transparent, flexible, and acetate substrates with platinum oxide thin film forming the active layer (8.0 mm x 60 mu m) for DNA detection. The sensor operated on the principle of conductance change resulting from the change in charge carrier density due to attachment of DNA to the platinum oxide surface. The DNA attachment onto platinum oxide was experimentally verified by performing Fourier transform infrared spectroscopy and optical fluorescence measurements. The binding constant of DNA to platinum oxide was found to be 7.35 pM for every percentage increase in fluorescence intensity. The sensor arrays showed a DNA concentration-dependent current change that was linear over a large dynamic range and sensitivity down to 0.5 nM. The label-free platinum oxide DNA sensors showed reproducibility with a coefficient of variation of less than 10%.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC, 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA
Department/Centre: ?? OS-CNSE ??
Depositing User: Id for Latest eprints
Date Deposited: 07 Oct 2017 06:08
Last Modified: 07 Oct 2017 06:08
URI: http://eprints.iisc.ac.in/id/eprint/57962

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