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Protein Protein Interaction Probed by Label-free Second Harmonic Light Scattering: Hemoglobin Adsorption on Spectrin Surface as a Case Study

Mishra, Kamini and Chakrabarti, Abhijit and Das, Puspendu K (2017) Protein Protein Interaction Probed by Label-free Second Harmonic Light Scattering: Hemoglobin Adsorption on Spectrin Surface as a Case Study. In: JOURNAL OF PHYSICAL CHEMISTRY B, 121 (33). pp. 7797-7802.

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Official URL: http://doi.org/10.1021/acs.jpcb.7b04503

Abstract

In this article, we have studied the binding of different naturally occurring hemoglobin (Hb) variants on erythrocyte skeletal protein, spectrin surface using the label free nondestructive second harmonic light scattering (SHLS) technique in aqueous buffer. Hemoglobin variants like sickle hemoglobin (HbS) and hemoglobin E (HbE) Were chosen as they associate with sickle cell disease and HbE beta-thalassemia, respectively, and their interaction with spectrin is compared with normal adult hemoglobin (HbA). The concentration dependent change in the Second harmonic light intensity from nanomolar spectrin solution has been measured after addition of small aliquots of hemoglobins. From the second harmonic titration data, the binding constant is calculated using a modified Langmuir adsorption model of hemoglobin binding to the spectrin surface. Interestingly, it is found that the binding constant for HbE (13.8 X 10(8) M-1) is 1 order of magnitude higher than that of HbS (1.6 X 10(8) M-1) or HbA (2.1 x 10(8) M-1) which indicates higher affinity of HbE for spectrin compared to HbA and HbS. The number of the Hb molecules bound to the spectrin surface was estimated to be of the order of hundred's which, is determined for the :first time.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
Department/Centre: Division of Chemical Sciences > Inorganic & Physical Chemistry
Depositing User: Id for Latest eprints
Date Deposited: 23 Sep 2017 05:06
Last Modified: 23 Sep 2017 05:06
URI: http://eprints.iisc.ac.in/id/eprint/57868

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