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Rapid, label-free CD4 testing using a smartphone compatible device

Kanakasabapathy, Manoj Kumar and Pandya, Hardik J and Draz, Mohamed Shehata and Chug, Manjyot Kaur and Sadasivam, Magesh and Kumar, Shreya and Etemad, Behzad and Yogesh, Vinish and Safavieh, Mohammadali and Asghar, Waseem and Li, Jonathan Z and Tsibris, Athe M and Kuritzkes, Daniel R and Shafiee, Hadi (2017) Rapid, label-free CD4 testing using a smartphone compatible device. In: LAB ON A CHIP, 17 (17). pp. 2910-2919.

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Official URL: http://doi.org/10.1039/c7lc00273d

Abstract

The most recent guidelines have called for a significant shift towards viral load testing for HIV/AIDS management in developing countries; however point-of-care (POC) CD4 testing still remains an important component of disease staging in multiple developing countries. Advancements in micro/nanotechnologies and consumer electronics have paved the way for mobile healthcare technologies and the development of POC smartphone-based diagnostic assays for disease detection and treatment monitoring. Here, we report a simple, rapid (30 minutes) smartphone-based microfluidic chip for automated CD4 testing using a small volume (30 mu L) of whole blood. The smartphone-based device includes an inexpensive (<$5) cell phone accessory and a functionalized disposable microfluidic device. We evaluated the performance of the device using spiked PBS samples and HIV-infected and uninfected whole blood, and compared the microfluidic chip results with the manual analysis and flow cytometry results. Through t-tests, Bland-Altman analyses, and regression tests, we have shown a good agreement between the smartphone-based test and the manual and FACS analysis for CD4 count. The presented technology could have a significant impact on HIV management in developing countries through providing a reliable and inexpensive POC CD4 testing.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Department/Centre: Division of Electrical Sciences > Electronic Systems Engineering (Formerly Centre for Electronic Design & Technology)
Depositing User: Id for Latest eprints
Date Deposited: 16 Sep 2017 06:26
Last Modified: 16 Sep 2017 06:26
URI: http://eprints.iisc.ac.in/id/eprint/57789

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