Pandya, Urja and Prakash, Sunita and Shende, Kishor and Dhuldhaj, Umesh and Saraf, Meenu (2017) Multifarious allelochemicals exhibiting antifungal activity from Bacillus subtilis MBCU5. In: 3 BIOTECH, 7 .
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A potential antagonist, designated strain Bacillus subtilis MBCU5 was previously isolated from vermicompost-amended soils of Gandhinagar, Gujarat, India. Crude allelochemicals from strain MBCU5 displayed strong antifungal activity against Macrophomina phaseolina as well as Rhizoctonia solani. These crude allelochemicals were tentatively identified as iturin, fengycin and surfactin through TLC and HPTLC analysis. Lipopeptides produced by MBCU5 were identified by MALDI-TOF-MS and LC-ESI-MS/MS analysis showed that iturin homologues (m/z 1020-1120), surfactin (m/z 1008.7 and m/z 1022.7), fengycin A and fengycin B (m/z 1400-1550) types of allelochemicals which are responsible for antifungal activity against pathogens. PCR analysis showed presence of genes (i.e. Iturin A synthetase KJ531680 and Surfactin synthetase KJ601726) involved in the biosynthesis of allelochemicals. Many reports showed lipopeptides from Bacillus species; this is the first report executed of multifarious allelochemicals from vermicompost-amended soil due to the presence of predominant Bacillus species.
| Item Type: | Journal Article |
|---|---|
| Publication: | 3 BIOTECH |
| Additional Information: | Copy right for this article belongs to the SPRINGER HEIDELBERG, TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY |
| Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit |
| Date Deposited: | 29 Jul 2017 10:26 |
| Last Modified: | 29 Jul 2017 10:26 |
| URI: | http://eprints.iisc.ac.in/id/eprint/57515 |
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