Simultaneous multiplane imaging-based localization encoded (SMILE) microscopy for super-resolution volume imaging

Mondal, Partha Pratim (2017) Simultaneous multiplane imaging-based localization encoded (SMILE) microscopy for super-resolution volume imaging. In: MICROSCOPY RESEARCH AND TECHNIQUE, 80 (4). pp. 333-337.

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Abstract

We propose a widefield-based rapid super-resolution volume imaging technique. This technique requires encoding single molecules to their respective planes and subsequent identification of the locus of individual molecule (both in the focal plane and off-focal planes). Experimentally, this is achieved by precise calibration of system PSF size and its natural spread in the off-focal planes using sub-diffraction fluorescent beads. The specimen plane touching the coverslip is chosen as the focal plane whereas planes far from coverslip (situated at large penetration depths) represent off-focal planes. The identification and sorting of single molecules are carried out by setting multiple cut-offs to the respective PSFs and a 3D super-resolved volume image is reconstructed. SMILE microscopy technique eliminates the need for multiple z-plane scanning, minimizes radiation-dose and enables rapid super-resolution volume imaging.

Item Type:	Journal Article
Publication:	MICROSCOPY RESEARCH AND TECHNIQUE
Publisher:	WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Additional Information:	Copy right for this article belongs to the WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Department/Centre:	Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	20 May 2017 04:55
Last Modified:	20 May 2017 04:55
URI:	http://eprints.iisc.ac.in/id/eprint/56871

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