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Indicator-Impregnated Agarose Films for Colorimetric Measurement of pH

Pathak, Abhishek and Borana, Jayant and Adhikari, Jayesh Vasudeva and Gorthi, Sai Siva (2017) Indicator-Impregnated Agarose Films for Colorimetric Measurement of pH. In: SLAS TECHNOLOGY, 22 (1). pp. 81-88.

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Official URL: http://dx.doi.org/10.1177/2211068216669686


In this article, we report on the development of indicator-impregnated agarose sensor films for colorimetric estimation of the pH of different standard buffer solutions and urine samples. In contrast to the conventional paper-based dipstick method, the presented method employs dried agarose that was infused with a chemical indicator, which when exposed to the test sample results in an appropriate chromogenic outcome. The color change was then quantified by the smart camera system. The divulged instrument has been calibrated with standard buffers and also tested with clinical urine samples. The systematic study was conducted to characterize the performance of the presented technique, such as interplate variations, time variations, volume variations, and shelf life study of agarose sensor films for the commercial viability of such a technique. The employed method is competent enough to bring a change in the paradigm of dipstick method from being qualitative estimation to quantitative, manual detection to automated, and a subjective approach of diagnosis to an objective one. The technique presented here has potential application in the field of point-of-care diagnostics with high fidelity, multiplexing, and robust quantitative estimation.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the SAGE PUBLICATIONS LTD, 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND
Department/Centre: Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Depositing User: Id for Latest eprints
Date Deposited: 21 Mar 2017 09:39
Last Modified: 21 Mar 2017 09:39
URI: http://eprints.iisc.ac.in/id/eprint/56409

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