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Automated Blood Sample Preparation Unit (ABSPU) for Portable Microfluidic Flow Cytometry

Chaturvedi, Akhil and Gorthi, Sai Siva (2017) Automated Blood Sample Preparation Unit (ABSPU) for Portable Microfluidic Flow Cytometry. In: SLAS TECHNOLOGY, 22 (1). pp. 73-80.

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Official URL: http://dx.doi.org/10.1177/2211068216663604


Portable microfluidic diagnostic devices, including flow cytometers, are being developed for point-of-care settings, especially in conjunction with inexpensive imaging devices such as mobile phone cameras. However, two pervasive drawbacks of these have been the lack of automated sample preparation processes and cells settling out of sample suspensions, leading to inaccurate results. We report an automated blood sample preparation unit (ABSPU) to prevent blood samples from settling in a reservoir during loading of samples in flow cytometers. This apparatus automates the preanalytical steps of dilution and staining of blood cells prior to microfluidic loading. It employs an assembly with a miniature vibration motor to drive turbulence in a sample reservoir. To validate performance of this system, we present experimental evidence demonstrating prevention of blood cell settling, cell integrity, and staining of cells prior to flow cytometric analysis. This setup is further integrated with a microfluidic imaging flow cytometer to investigate cell count variability. With no need for prior sample preparation, a drop of whole blood can be directly introduced to the setup without premixing with buffers manually. Our results show that integration of this assembly with microfluidic analysis provides a competent automation tool for low-cost point-of-care blood-based diagnostics.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the SAGE PUBLICATIONS LTD, 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND
Department/Centre: Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Depositing User: Id for Latest eprints
Date Deposited: 21 Mar 2017 09:25
Last Modified: 21 Mar 2017 09:25
URI: http://eprints.iisc.ac.in/id/eprint/56408

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