ERK activated by Histamine H1 receptor is anti-proliferative through spatial restriction in the cytosol

Jain, Ruchi and Watson, Uchenna and Saini, Deepak Kumar (2016) ERK activated by Histamine H1 receptor is anti-proliferative through spatial restriction in the cytosol. In: EUROPEAN JOURNAL OF CELL BIOLOGY, 95 (12). pp. 623-634.

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Abstract

Histamine, a primary mediator of allergic responses, elicits its effects by activating specific receptors belonging to the GPCR family in target cells. Activation of histamine receptor can activate MAP kinases as recorded by monitoring the phosphorylation of extracellular signal regulated kinase (ERK). Despite this, ERK phosphorylation does not translate into pro-proliferative changes after histamine stimulation in HeLa cells. Here we show that histamine H1 receptor activation mediates MAPK activation through PLC beta, Src, PKC delta and MEK pathway, but does not lead to nuclear relocalization of phospho-ERK (pERK), classically associated with pro-proliferative changes. Live cell imaging, FRET and FRAP measurements along with functional analysis reveal that pERK generated by histamine activation is physically and functionally restricted in the cytosol and the findings report a spatial regulation of MAPK cascade activated non canonically through GPCRs unlike its canonical activation by EGF. (C) 2016 Elsevier GmbH. All rights reserved.

Item Type:	Journal Article
Publication:	EUROPEAN JOURNAL OF CELL BIOLOGY
Additional Information:	Copy right for this article belongs to the ELSEVIER GMBH, URBAN & FISCHER VERLAG, OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	31 Jan 2017 05:28
Last Modified:	31 Jan 2017 05:28
URI:	http://eprints.iisc.ac.in/id/eprint/56120

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