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Supramolecular Approach to Enzyme Sensing on Paper Discs Using Lanthanide Photoluminescence

Gorai, Tumpa and Maitre, Uday (2016) Supramolecular Approach to Enzyme Sensing on Paper Discs Using Lanthanide Photoluminescence. In: ACS SENSORS, 1 (7). pp. 934-940.

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Official URL: http://dx.doi.org/10.1021/acssensors.6b00341

Abstract

A paper based photoluminescent biosensor has been developed, with green Tb-luminescence as the output, for the rapid detection of beta-glucosidase and lipase, both in purified form and in biological/natural fluids. A Tb Cholate hydrogel doped with specially designed, specific enzyme substrates (''pro''-sensitizers) was integrated on filter paper discs. The addition of small volumes(similar to 5 mu L) of enzyme solutions on these discs led to enhanced green emission (UV lamp, lambda(ex) 365 nm), allowing qualitative detection of the enzymes-when needed, the intensity enhancement can be quantified using image processing software, or for multiple samples using a commercial plate reader. This simple technique allowed the detection of beta-glucosidase in almond extract and lipase in blood serum. Easy identification of the inhibition of beta-glucosidase was also demonstrated. The paper based sensor is inexpensive, user-friendly and needs low volumes of biological sample for analysis. This strategy has the potential to be useful for possible clinical applications.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
Department/Centre: Division of Chemical Sciences > Organic Chemistry
Depositing User: Id for Latest eprints
Date Deposited: 03 Dec 2016 09:41
Last Modified: 03 Dec 2016 09:41
URI: http://eprints.iisc.ac.in/id/eprint/55361

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