Hyperglycosylation of glycopeptidolipid of Mycobacterium smegmatis under nutrient starvation: structural studies

Mukherjee, Raju and Gomez, Manuel and Jayaraman, Narayanaswamy and Smith, Issar and Chatterji, Dipankar (2005) Hyperglycosylation of glycopeptidolipid of Mycobacterium smegmatis under nutrient starvation: structural studies. In: Microbiology, 151 (7). pp. 2385-2392.

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Abstract

The presence of a polar species of glycopeptidolipid (GPL) in carbon-starved Mycobacterium smegmatis has been reported previously. In this study, the complete structure of this GPL is established with the help of MALDI-TOF (matrix assisted laser desorption/ionization time of flight) and ESI (electrospray ionization) -MS, ${^1^3}C-SEFT$ (spin echo Fourier transform) -NMR spectroscopy, and HPLC analysis. In the molecule, two units of a 3,4-di-O-methyl derivative of rhamnose are attached to L-alaninol via a 1-->2 linkage. Various methyl derivatives of rhamnose and 6-deoxytalose were synthesized as standards to establish this structure. The accumulation of this polar GPL in M. smegmatis is sigB dependent, as a SigB-overproducing strain of M. smegmatis shows the presence of this spot in the exponential phase, and a sigB-knockout strain of M. smegmatis does not show the presence of any polar GPLs.

Item Type:	Journal Article
Publication:	Microbiology
Publisher:	Society for General Microbiology
Additional Information:	The Copyright belongs to Society for General Microbiology.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit Division of Chemical Sciences > Organic Chemistry
Date Deposited:	23 Mar 2006
Last Modified:	19 Sep 2010 04:23
URI:	http://eprints.iisc.ac.in/id/eprint/5512

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