Gelatin Methacrylate Hydrogels as Biomimetic Three-Dimensional Matrixes for Modeling Breast Cancer Invasion and Chemoresponse in Vitro

Arya, Anuradha D and Hallur, Pavan M and Karkisaval, Abhijith G and Gudipati, Aditi and Rajendiran, Satheesh and Dhavale, Vaibhav and Ramachandran, Balaji and Jayaprakash, Aravindakshan and Gundiah, Namrata and Chaubey, Aditya (2016) Gelatin Methacrylate Hydrogels as Biomimetic Three-Dimensional Matrixes for Modeling Breast Cancer Invasion and Chemoresponse in Vitro. In: ACS APPLIED MATERIALS & INTERFACES, 8 (34). pp. 22005-22017.

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Abstract

Recent studies have shown that three-dimensional (3D) culture environments allow the study of cellular responses in a setting that more closely resembles the in vivo milieu. In this context, hydrogels have become popular scaffold options for the 3D cell culture. Because the mechanical and biochemical properties of culture. matrixes influence crucial cell behavior, selecting a suitable Matrix for replicating in vivo cellular phenotype in vitro is essential for understanding disease progression. Gelatin methacrylate (GelMA) hydrogels have been the focus of much attention because of their inherent bioactivity, favorable hydration and diffusion properties, and ease-of-tailoring of their physicochemical characteristics. Therefore, in this study we examined the efficacy of GelMA hydrogels as a suitable platform to model specific attributes of breast canter. We observed increased invasiveness in vitro and increased tumorigenic ability in vivo in breast cancer cells cultured on GelMA hydrogels. Further, cells cultured on GelMA matrixes were more resistant to paclitaxel treatment, as shown by the results of cell-cycle analysis and gene expression. This study, therefore, validates GelMA hydrogels as inexpensive, cell-responsive 3D platforms for modeling key characteristics associated with breast cancer Metastasis, in vitro.

Item Type:	Journal Article
Publication:	ACS APPLIED MATERIALS & INTERFACES
Additional Information:	Copy right for this article belongs to the AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
Department/Centre:	Division of Mechanical Sciences > Mechanical Engineering
Date Deposited:	28 Oct 2016 06:53
Last Modified:	28 Oct 2016 06:53
URI:	http://eprints.iisc.ac.in/id/eprint/55014

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