Rai, Mamta and Katti, Prasanna and Nongthomba, Upendra (2016) Spatio-temporal coordination of cell cycle exit, fusion and differentiation of adult muscle precursors by Drosophila Erect wing (Ewg). In: MECHANISMS OF DEVELOPMENT, 141 . pp. 109-118.
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Abstract
The mechanisms of cell cycle exit by myoblasts during skeletal muscle development are poorly understood. Cell cycle arrest is known to be a prerequisite for myoblast fusion and subsequent differentiation. Despite tremendous knowledge on myoblast fusion and differentiation, tissue-specific factors that spatio-temporally regulate the cell cycle exit are not well known. In this paper, we show that the transcriptional factor/co-activator ``Erect wing'' (Ewg) synchronises myoblast cell cycle exit with that of the fusion process. Ewg-null myoblasts show delayed temporal development of dorsal longitudinal muscles (DLMs), a group of indirect flight muscles (IFMs), which culminates to abnormal and asymmetric muscle pattern. A role for Ewg in cell cycle exit at G1/S stage is also shown. Reducing Cyclin E dose in Ewg-null mutant rescues the lack of IFMs and flight ability. Thus, we show that Ewg repression of Cyclin E expression is required for the arrest of myoblast proliferation and initiate myoblast fusion and terminal differentiation. (C) 2016 Elsevier Ltd. All rights reserved.
Item Type: | Journal Article |
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Publication: | MECHANISMS OF DEVELOPMENT |
Additional Information: | Copy right for this article belongs to the ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics |
Date Deposited: | 22 Oct 2016 06:48 |
Last Modified: | 22 Oct 2016 06:48 |
URI: | http://eprints.iisc.ac.in/id/eprint/54655 |
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