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Automated quantitative cytological analysis using portable microfluidic microscopy

Jagannadh, Veerendra Kalyan and Murthy, Rashmi Sreeramachandra and Srinivasan, Rajesh and Gorthi, Sai Siva (2016) Automated quantitative cytological analysis using portable microfluidic microscopy. In: JOURNAL OF BIOPHOTONICS, 9 (6). pp. 586-595.

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Official URL: http://dx.doi.org/10.1002/jbio.201500108

Abstract

In this article, a portable microfluidic microscopy based approach for automated cytological investigations is presented. Inexpensive optical and electronic components have been used to construct a simple microfluidic microscopy system. In contrast to the conventional slide-based methods, the presented method employs microfluidics to enable automated sample handling and image acquisition. The approach involves the use of simple in-suspension staining and automated image acquisition to enable quantitative cytological analysis of samples. The applicability of the presented approach to research in cellular biology is shown by performing an automated cell viability assessment on a given population of yeast cells. Further, the relevance of the presented approach to clinical diagnosis and prognosis has been demonstrated by performing detection and differential assessment of malaria infection in a given sample.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the WILEY-V C H VERLAG GMBH, POSTFACH 101161, 69451 WEINHEIM, GERMANY
Keywords: diagnostic imaging; cytodiagnosis; microfluidics; point of care systems; cell survival; microfluidic microscopy
Department/Centre: Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Depositing User: Id for Latest eprints
Date Deposited: 22 Aug 2016 09:46
Last Modified: 22 Aug 2016 09:46
URI: http://eprints.iisc.ac.in/id/eprint/54496

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