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Curtailed Light Sheet Microscopy for Rapid Imaging of Macroscopic Biological Specimens

Rasmi, Chelur K and Madhangi, Mani and Nongthomba, Upendra and Mondal, Partha Pratim (2016) Curtailed Light Sheet Microscopy for Rapid Imaging of Macroscopic Biological Specimens. In: MICROSCOPY RESEARCH AND TECHNIQUE, 79 (6). pp. 455-458.

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Official URL: http://dx.doi.org/10.1002/jemt.22665

Abstract

We study the feasibility of volume imaging from a few angular views/scans in a light sheet fluorescence microscopy. Two-dimensional (2D) images (angular views) were acquired at an angular separation of 108 and volume images were constructed with merely 18, 9, and 6 views. We study the structural changes in a 5-day old Zebrafish embryo labeled with Phalloidin TRITC that binds to F-Actin of embryo cell. To collect the data, the specimen is rotated (for varying sampling angles Du) with respect to a fixed vertical axis passing through the volume-of-interest (yolk sac). In the proposed realization of selective plane illumination microscopy (SPIM) technique, the translation is completely avoided. Analysis shows rich structural information with marginal reduction in contrast. Comparison with the state-of-the-art SPIM shows appreciable volume reconstruction (from an order less 2D scans) that may be good enough for rapid monitoring of macroscopic specimens. (C) 2016 Wiley Periodicals, Inc.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Keywords: fluorescence microscopy; light sheet microscopy; biological imaging
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Depositing User: Id for Latest eprints
Date Deposited: 30 Jun 2016 04:52
Last Modified: 03 Nov 2018 08:47
URI: http://eprints.iisc.ac.in/id/eprint/54087

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